An inflammatory reaction is induced after release of proinflammatory mediators such asinterleukin 1 and 6 and tumour necrosis factor ?. These mediators stimulate the liver tosuppress the syntheses of albumin and endure the syntheses of Acute phase protein forinstance C-reactive protein. The aim of this paper was to perform a method validation on animmune turbidimetric assay to quantify C-reactive protein in canine serum at the laboratory atSkara Animals Hospital, Skara, Sweden. The validation involved evaluation of the assaylinearity, precision, stability and recovery.The method was proved to be linear for both TruLab control and Medinor control.

The analysis of Acute phase protein serum amyloid A (SAA) has recently been brought into clinical use in veterinary medicine. Some of the difficulties with incorporating the SAA method in clinical practice have been the expensive and rather large equipment required for the method. Due to these difficulties only larger clinics can afford to use the SAA analysis.The company Equinostic has recently developed a smaller instrument that costs one-tenth of a larger instrument. The instrument is named EVA1 and has so far only been used to analyze SAA in horses.The aim of this study was to investigate if the EVA1 instrument could be used to analyze SAA in cats. This study included 24 serum samples from cat, which were first analyzed twice on the EVA1 instrument and then sent to the Strömsholm Referral Animal Hospital in Sweden where they reanalyzed the samples using a validated reference method.

Haptoglobin is an Acute phase protein with important biological role because of its capacity to bind to haemoglobin. Haptoglobin exists in three major genetic polymorphism types: Hp1-1, Hp2-1 and Hp2-2, the distribution of which has been associated with abdominal aortic aneurysm (AAA), an asymptomatic aortic disease common among men older than 65 years.    Five different electrophoretic methods were tested according to their ability to separate the haptoglobin phenotypes. The detection was based on a produced hemolysate of blood in which haemoglobin binds to haptoglobin thereby forming a complex that can be detected by specific haemoglobin staining using TMB-dihydrochloride and hydro peroxide as substrate resulting in an azure-green color of the bands. Samples from 15 patients who had suffered surgery for not broken AAA, that is more than5.0 cmaortic diameter, and 15 samples from matched controls were analyzed.    Among the five tested electrophoretic methods best migration and separation was seen on the pre-cast agarosgel Hydragel HR on the instrument Hydrasys. The other four methods gave less successful results.

The concentration of Acute phase proteins in serum is altered as a response to inflammation, infection or trauma. this makes these proteins potential biomarkers of unspecific pathological processes. In cattle, haptoglobin and serum amyloid A are the two Acute phase proteins that show the largest increase in serum concentration as a result of inflammatory processes. The haptoglobin concentration has been shown to correlate to clinical signs as well as inflammatory processes found at post mortem examination. This feature could be useful prognosis of the individual as well as for monitoring the health status of a herd or identifying carcasses that shold be examined closer at the slaughter-house.

The cell cycle is the time a cell spends between two cell divisions. The cell cycle includes several parallel processes, all of which must be completed before a cell is mature for dividing. In the first place, all subcomponents (RNA, protein and membrane lipids) need to double in quantity and this occurs continuously through the cell cycle. Furthermore, the genome and some chromosomal proteins must double and this take place during a limited interval in the middle of the cell cycle (S-phase). On either side of the S-phase are two "gaps" which is called G1 and G2. When the cell has passed a point in the G-phase they are irreversibly programmed to progress through the remaining of the cell cycle and will undergo the next cell division. When a cell have passed a point in the G1 phase, approximately four hours after mitosis, a normal cell is in a state of indecision.

In studies of serum, in the early 1930s, from patients with pneumonia, a factor was found. It could agglutinate certain pneumococcal species. This factor, which later became known as C-reactive protein (CRP), increased sharply during the early and middle stages of the disease. CRP was identified as an Acute phase protein and found especially in bacterial infections. CRP is synthesized in the liver by stimulation of interleukin 6 (IL-6), which is produced by the monocytes, and consists of five non-covalently bound subunits.The aim of this study was to compare two different analytical instruments (Vitros 5.1 FS Ortho-Clinical Diagnostics and QuikRead CRP Orion Diagnostica) for CRP analysis.

Bovine Respiratory Syncytial Virus (BRSV) is a common cause of respiratory disease among young cattle. The virus causes severe losses; the herd mortality rate can sometimes be as high as 20 %. In this study the avidity (i.e the antigen binding force) of BRSV specific antibodies was measured to see if there was a difference between antibodies produced during an acute phase of infection and antibodies produced by earlier infected animals. A commercially available ELISA-testkit against BRSV-specific antibodies was used and an incubation step with 6M urea was added. The effect of the urea is that it breaks the weak bonds between antibodies and antigen while the stronger bonds remain intact. Four different groups of animals were included in this study; seven calves that were naturally infected, three acutely infected calves with known time of infection, five cows that were seropositive during several years and four experimentally infected calves that had been a part of a vaccine trial. The results of this study showed that antibodies produced during the acute phase of an infection had a low avidity and that the avidity increased with time after infection.

This thesis investigates three different surface modifications, and the route to design and synthesize them. The thesis is therefore divided into three sub- projects. (i.) Design and synthesis of a peptide which secondary structure could be controlled by a negatively charged surface. (ii.) Design and synthesis of a cyclic peptide, that would self-organize prior to surface interaction, using the type I anti-freeze protein of a winter flounder as template. (iii.) The use of solid-phase synthesis to make the synthesis of SAM-molecules easier..

In this paper the authors are focusing on people that work in psychiatric acute care. How do they identify the individual that is in most need of acute care? How do they judge in this kind of cases?    The foundation in this study is in the qualitative method. The material to this study has been collected by doing qualitative interviews with six people in different professions and positions in psychiatric acute care.   The material that we collected from these six qualitative interviews has been analysed with help from Sherif?s and Hovland?s (1961) Social judgement theory.

I denna uppsats behandlas de grammatiska fenomenen complete phase och incomplete phase i det austronesiska språket rotumanska. Min avsikt med uppsatsen är att ta reda på varför complete phase respektive incomplete phase används i en viss syntaktisk eller morfologisk kontext. Jag har baserat denna studie endast på litteratur, främst från C.M Churchwards Rotuman Grammar and Dictionary..

The development of quantitative, real-time PCR (qPCR) combined with the mapping of the canine genome opens new possibilities in veterinary medicine. This method provides a quick and accurate quantification of the expression of a specific gene at a given point in time and thereby also information of how the gene expression for a certain protein is influenced by various conditions and diseases. One possible area of application is identifying bio markers for cancer. Recently the protein serglycin (the core protein of an intracellular proteoglycan) was found to act as a selective marker for the disease acute myeloid leukemia in humans (Niemann et al, 2007). Serglycin is produced by most hematopoietic cells, although mast cells account for the largest amount of serglycin.

Laminitis is a common but devastating condition, and affected horses are often euthanized or influenced a very long time. Many horses never return to their original capacity. Laminitis is often associated with severe pain and a long time of convalescence. Symthoms includes lameness, hoof hyperthermia, strenghthened digital pulse and pain. The horse often tries to move the body weight away from the forelegs which are often more severly affected than the hindlimbs.

The aim of this study was to investigate the intraoperative surgical stress response in dogs undergoing ovariohysterectomy and to compare acepromazine and medetomidine as premedications. 15 dogs diagnosed with pyometra were used in this study. Blood pressure and heart rate were used as parameters to measure surgical stress response. The surgery was divided into four phases. Phase 0 was the period 10 minutes before the skin incision, phase 1 was skin incision and opening of abdomen, phase 2 was manipulation of uterine horns, ligation and transection of mesovarium and phase 3 was ligation of cervix, removal of organs and closing the abdomen. The results showed that phase 2 was the most intense phase of surgical stress, regardless of which premedication the dogs received. When acepromazine and medetomidine were compared the results showed that within phase 3, all dogs that were given medetomidine had higher blood pressure compared to dogs that were given acepromazine. The dogs that were given acepromazine had higher heart rate than the dogs that were given medetomidine in phase 0, phase 2 and phase 3. .

Abstract:In previous experiments with the bacterium Ideonella dechloratans a probable sequencecoding for a cytochrome c protein (cyt c) was observed, the sequence is assumed to have afunction in the bacterial respiratory chain. Detection attempts have been made to try to findthis protein, but have not succeeded. The purpose of this project is to detect the expressionof mRNA associated with the cytochrome c. And if it is detected, it will also be investigated ifthere is any difference in expression of the mRNA due to aerobic or anaerobic environment.Total RNA was purified from cultures grown under aerobic and anaerobic conditions. cDNAwas then synthesized using reverse transcriptase and subsequently amplified with thespecific primers in a qRT-PCR.qRT-PCR testing showed a distinct amplification of primer product, confirming that I.dechloratans expresses mRNA coding for the cyt c protein.

Bovine viral diarrhoea virus (BVDV) is the causative agent of a complex of disease syndromes in cattle with high economical and welfare impacts. BVDV occurs as two biotypes; cytopathic (BVDVcp) and noncytopathic (BVDVncp) determined by differential effects on cultured cells and can also be divided into two genotypes (BVDV1 and BVDV2) on the basis of genomic diversity. The interaction between BVDV and the host?s immune system is regarded a key aspect in the sequel of BVDV infection. Infection with BVDV normally causes an acute transient infection, with mild to subclinical signs, but occasionally results in severe and even fatal disease.